Objective: To analyze the effect of application cases and scenario simulation teaching methods in clinical nursing teaching. Methods: A total of 88 nursing interns and 11 nurses in the department who interned in our hospital from January 2021 to April 2023 were randomly divided into control group and observation group, with 44 nurses in each group, the control group was given regular teaching, the observation group was given case and scenario simulation teaching method, and the nurses in the department carried out training, and the assessment results, self-learning ability score and work ability score of nurses before and after training of the two groups were compared. Results: Compared with the control group, the interns in the observation group had higher assessment scores and independent learning ability scores (P<0.05). Nurses in the department scored higher after training than before training (P<0.05). Conclusion: The implementation of case and scenario simulation teaching method in clinical nursing teaching plays an important role in improving the independent learning ability of interns, and can also strengthen the working ability of nurses in the department, so it can be popularized and applied.